| 同型半胱氨酸对血管抗氧化酶基因表达的影响 |
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| 引用本文： | 单妍,张敏华,张明,田兴亚. 同型半胱氨酸对血管抗氧化酶基因表达的影响[J]. 云南师范大学学报(自然科学版), 2011, 31(3): 64-67 |
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| 作者姓名： | 单妍 张敏华 张明 田兴亚 |
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| 作者单位： | 昆明医学院海源学院,生物化学教研室,云南,昆明,650106 |
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| 基金项目： | 昆明医学院海源学院科研基金资助项目 |
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| 摘 要： | 目的探讨同型半胱氨酸(Hcy)诱导抗氧化酶基因表达的影响。方法采用实时定量RT-PCR测定基因的表达,用DCF—DA荧光探针测定细胞内ROS;研究材料为HUEVC及高Hcy小鼠胸主动脉;结果同型半胱氨酸能诱导人脐静脉血管内皮细胞(HUEVC)的ROS水平升高,抑制HUEVC的谷胱甘肽过氧化物酶(GSHPx)和过氧化氢酶(CAT)基因的表达。在高同型半胱氨酸血症小鼠胸主动脉中,谷胱甘肽过氧化物酶、过氧化氢酶和锰超氧化物歧化酶(Mn-SOD)基因表达均明显降低。结论 Hcy能促进上皮细胞内活性氧的产生,可明显促进氧化损伤,同时显著降低抗氧化酶(谷胱甘肽过氧化物酶、过氧化氢酶及锰超氧化物歧化酶)的活性。
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| 关 键 词： | 同型半胱氨酸 血管内皮细胞 活性氧物质 抗氧化酶 |
Effect of Homocysteine on The Expression of Endothelial Antioxidative Enzymes in Vein |
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| SHAN Yan,ZHANG Min-hua,ZHANG Ming,TIAN Xing-ya. Effect of Homocysteine on The Expression of Endothelial Antioxidative Enzymes in Vein[J]. Journal of Yunnan Normal University (Natural Sciences Edition), 2011, 31(3): 64-67 |
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| Authors: | SHAN Yan ZHANG Min-hua ZHANG Ming TIAN Xing-ya |
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| Affiliation: | SHAN Yan,ZHANG Min-hua,ZHANG Min,TIAN Xing-ya (Dept.of Biochemistry,School of Hai Yuan,Kunming Medical University,Kunming 650031,China) |
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| Abstract: | To study the effects of homocysteine(Hcy) at different concentrations on the expression of antioxidative enzymes in cultured HUEVC and in aorta of mice with hyperhomocysteinemia.Methods: The expression of tested genes was detected by realtime RT-PCR.The levels of ROS in cells were measured by using DCF-DA as a fluorescence probe;Research material for the HUEVC and the aorta of mice with hyperhomocysteinemia.Results:Homocysteine promoted the levels of ROS and down-regulated the expression of catalase and glu... |
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| Keywords: | Homocysteine Vascular endothelial cell Reactive oxygen species Antixoidant enzyme |
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