| 机械刺激对烟草悬浮培养细胞耐盐和重金属胁迫的影响 |
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| 引用本文： | 李忠光,杨仕忠,谢虹.机械刺激对烟草悬浮培养细胞耐盐和重金属胁迫的影响[J].云南师范大学学报(自然科学版),2011,31(4). |
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| 作者姓名： | 李忠光 杨仕忠 谢虹 |
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| 作者单位： | 1. 云南师范大学 生命科学学院,云南昆明 650092;生物能源持续开发利用教育部工程研究中心,云南昆明 650092;云南省生物质能与环境生物技术重点实验室,云南昆明 650092
2. 云南师范大学 生命科学学院,云南昆明 650092;生物能源持续开发利用教育部工程研究中心,云南昆明 650092 |
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| 基金项目： | 云南省自然科学基金资助项目，云南省教育厅基金资助项目 |
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| 摘 要： | 机械刺激(mechanical stimulation,MS)是自然界中普遍存在但又易被忽视的一种非生物胁迫因子.用提高摇床转速的方式对烟草悬浮细胞施加机械刺激,发现机械刺激可提高烟草悬浮培养细胞在盐和重金属胁迫下的存活率和再生能力,缓解盐和重金属胁迫下的细胞活力丧失和丙二醛( malonaldehyde,MDA)含量的积累.这些结果表明机械刺激可诱导烟草悬浮细胞耐盐和重金属胁迫耐性的形成.
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| 关 键 词： | 烟草悬浮培养细胞 机械刺激 耐盐性 重金属胁迫耐性 |
Effect of Mechanical Stimulation on Salt and Heavy Metal Tolerance in Tobacco Suspension Cultured Cells |
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| LI Zhong-Guang,YANG Shi-zhong,XIE Hong.Effect of Mechanical Stimulation on Salt and Heavy Metal Tolerance in Tobacco Suspension Cultured Cells[J].Journal of Yunnan Normal University (Natural Sciences Edition),2011,31(4). |
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| Authors: | LI Zhong-Guang YANG Shi-zhong XIE Hong |
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| Institution: | LI Zhong-Guang1,2,3,YANG Shi-zhong1,XIE Hong1,2(1.School of Life Sciences,2.Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,3.Key Laboratory of Biomass Energy and Environmental Biotechnology,Yunnan Province,Yunnan Normal University,Kunming 650092,China) |
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| Abstract: | Mechanical stimulation(MS),existing widely but ignored usually in nature,is one of the abiotic stress factors.Here,tobacco suspension cells were subjected to mechanical stimulation by increase rotational speed in a shaker incubator,which found that mechanical stimulation could increase survival percentage and regrowth of tobacco suspension cells under salt and heavy metal stress,and alleviate the loss of cell vitality and accumulation of malonaldehyde(MDA) of tobacco suspension cells.These results suggested... |
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| Keywords: | tobacco suspension cells mechanical stimulation salt tolerance heavy metal tolerance |
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