空间诱变致成瘤性减弱的B16细胞株形态学观察

观察动物接种实验致瘤性显著减弱的空间诱变小鼠黑色素瘤B16细胞株的细胞形态、细胞骨架和表面结构与对照细胞株的差异。分别使用激光共聚焦显微镜观察筛选所得阳性细胞株与对照细胞株,以及用荧光素标记的鬼笔环肽染色,观察细胞骨架的异同。应用原子力显微镜(AFM)对筛选所得阳性细胞株和对照细胞株分别成像,观察空间诱变细胞株与对照组细胞株所得图像的差异。激光共聚焦显微镜观察结果显示,从空间诱变细胞株中筛选出的8#细胞株的细胞骨架荧光较对照组增强,细胞骨架纤维比对照组粗大。AFM图像显示,8#细胞株分泌的纤维粘连蛋白的纤维形态和分布与对照组细胞有较大差异,其纤维较为扁平、走行弯曲或呈轻度迂曲状;对照组细胞纤维呈山脊状、放射状,源自细胞伪足部细胞膜。在8#细胞株图像中有少量“孔洞”样结构,而在对照组细胞中未观察到类似结构。

Morphological Study on the Low Oncogenesis B16 Cell Clone Mutagenized by Space Environment

The morphological characters of the low oncogenesis melanoma B16 cell clone mutagenized by space environment are studied.The mutated cell clones and control B16 cells were detected with immunofluorescence cytochemistry and confocal lasers scanning microscopy.The mutated cell clones and the control cell clones were imaged by AFM,and the differences between their images were observed.The fluorescent intensity of microfilaments increases in 8# cells.The fiber of cytoskeleton in 8# cells is enlarged.The AFM images show that the shape of the fibronectin secreted by 8# cell clone is different from that of the control clone,the fiber is flatter than that of the control clone,it is bent and circuity;while the fiber of the control clone is like mountain ridge radiated from the cell pseudopodia.There is a few "well" or "hole" like structures on the surface of the 8# cell clone,but there is no such structures observed in the control clone.The surface structure and the distribution of cell cycle are different from those of the control clone.Whether the space environment could increase the immunogenicity of the tumor cells remains to be studied.The findings suggest that there are mutagenesis factors which could mutate the culture tumor cells in the outer space.