| 一株噬甲烷菌的分离鉴定及其消除煤矿瓦斯能力的初步研究 |
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| 引用本文： | 段学军,黄春晓.一株噬甲烷菌的分离鉴定及其消除煤矿瓦斯能力的初步研究[J].科技导报(北京),2007,25(11):5-8. |
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| 作者姓名： | 段学军 黄春晓 |
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| 作者单位： | 中原工学院能源与环境学院 郑州450007 |
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| 基金项目： | 河南省自然科学基金项目(0611022000) |
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| 摘 要： | 借鉴滚管法,从煤矿矿井及甲烷处理水稻田土壤中分离纯化,获得一株可在以甲烷为唯一碳源的培养基上正常生长且具有较高甲烷氧化活性的噬甲烷细菌,暂命名为ZD4,经对该菌进行形态观察、Biolog生理生化分析、G Cmol%含量分析和16SrDNA序列同源性比较,鉴定该菌为甲烷甲基单胞菌(Methylomonas capsulatus),并对ZD4菌株进行了生长量、对甲烷消除作用的分析。试验结果表明,该菌株可以使甲烷含量在一周内比初始甲烷浓度降低85%以上。利用其良好的甲烷消除能力,用于煤矿瓦斯治理是可行的。
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| 关 键 词： | 甲烷甲基单胞菌 分离鉴定 甲烷消除 |
| 文章编号： | 1000-7857（2007）11-0005-04 |
| 修稿时间： | 2007-04-05 |
Separation and Identification of a Methane-oxidizing Bacteria and a Preliminary Study on Its Methane Oxidation Capacity |
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| DUAN Xuejun,HUANG Chunxiao.Separation and Identification of a Methane-oxidizing Bacteria and a Preliminary Study on Its Methane Oxidation Capacity[J].Science & Technology Review,2007,25(11):5-8. |
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| Authors: | DUAN Xuejun HUANG Chunxiao |
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| Institution: | Department of Energy and Environment Engineering, Zhongyuan Institute of Technology, Zhengzhou 450007, China |
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| Abstract: | A bacterium (strain ZD4) was separated from soil treated with methane, which can grow with methane as the sole carbon source. Based on the morphological observations and the analysises of Biolog-GN and G C mol% on the 16S rRNA gene, the strain was identified as Methylomonas capsulatus. The detection of the methane oxidation rate indicates that the strain ZD4 can reduce the methane concentration by 85%, and therefore, it is feasible to control the coal mine gas using ZD4's methane oxidation capacity. |
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| Keywords: | Methylomonas capsulatus isolation identification methane oxidation |
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