不同基因型小麦成熟胚盾片组织培养效果研究

不同基因型及其外植体的愈伤组织诱导和植株分化研究是建立高效植株再生和遗传转化系统的基本条件。文章首次以去胚的成熟胚盾片为外植体材料,对10个小麦基因型进行了离体操作。结果显示:愈伤组织诱导率平均可达96.5%,10个基因型的愈伤组织都能分化出绿苗,分化频率最高可达10.5%,没有白苗化现象;根的分化与绿点、绿苗的分化之间具有显著的相关性;鄂恩1号的植株再生能力显著优于其它基因型,其植株再生系统已具备了遗传转化的基本要求。

Screening wheat genotypes (Triticum aestivum) for high callus induction and regeneration capability from mature embryo cultures

The callus induction and the plant regeneration from the different genotype and its explant with the method of tissue culture are the important factors in establishing cell selection and genetic transformation systems.Mature embryo scutellum(without embryo) explants,of 10 Chinese wheat(Triticum aestivum) varieties were used to screen genotype to obtain high frequencies of callus induction and plant regeneration in vitro.It shows: the average callus induction rate can reach 96.5%;10 genotypes regenerated green plants;the genotype with a high regeneration capability was 10.5%;No genotypes produced albino plants;and the callus formation is independent from rooting,plant regeneration and green spot.There were significant correlations between rooting and green plants,rooting and green spots.