枸杞组织培养中形态发生的细胞组织学观察

Cytohistological Observations of Morphogenesis on Tissue Culture of Lycium barbarum L

The cutting pieces of leafves in Lycium barbarum L were cultured onMS media supplemented 0.5mg/16-BA and 0.5mg/NAA.The buds weredifferentiated after 25 days in culture and the regenerated plants formedafter 40 days.Cytohistological observations indicated that the dedifferentia-tion occurred primarily in the mesophyll cells near the cutting sites,thenthe middle mesophyll cells.After active dividing,a large number of calliwere obtained.Bud primordia were differentiated from calli dueto divisionsof external layer cells in calli.During this course the celellular DNAsynthesis was earlier than morphological changes.Dynamics of DNA synth-esis may be considered as an initial signal of cell morphological changes.It is also important to make localization of DNA and tracking study.