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65个菊花栽培品种遗传多样性的AFLP分析
引用本文:吴在生,李海龙,刘建辉,左志锐,田瑞昌. 65个菊花栽培品种遗传多样性的AFLP分析[J]. 南京林业大学学报(自然科学版), 2007, 31(5): 67-70
作者姓名:吴在生  李海龙  刘建辉  左志锐  田瑞昌
作者单位:西北农林科技大学园艺学院,陕西,杨陵,712100;张家港三利农业科技有限公司,江苏,张家港,215617;河南科技大学林业职业技术学院,河南,洛阳,471002;西北农林科技大学园艺学院,陕西,杨陵,712100;张家港三利农业科技有限公司,江苏,张家港,215617;济源市农业科学研究所,河南,济源,454652
摘    要:利用AFLP技术, 对65个菊花品种进行了遗传多样性分析.选用6对多态性高、分辨力强的引物组合分别对供试材料的基因组DNA进行扩增, 共获得244条清晰可辨的谱带, 其中多态性带178条, 多态位点百分率达72.95%,揭示了菊花栽培种质丰富的遗传多样性.对AFLP扩增结果采用UPGMA法进行聚类分析,多数瓣型相同的菊花种质表现出较为密切的亲缘关系,地域来源与聚类结果也有一定程度的相关性,而花色与聚类结果无明显的相关性.

关 键 词:菊花  遗传多样性  亲缘关系  AFLP
文章编号:1000-2006(2007)05-0067-04
修稿时间:2007-03-28

Analyses of Genetic Diversity Among 65 Chrysanthemum Cultivars Based on AFLP
WU Zai-sheng,LI Hai-long,LIU Jian-hui,ZUO Zhi-rui,TIAN Rui-chang. Analyses of Genetic Diversity Among 65 Chrysanthemum Cultivars Based on AFLP[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2007, 31(5): 67-70
Authors:WU Zai-sheng  LI Hai-long  LIU Jian-hui  ZUO Zhi-rui  TIAN Rui-chang
Abstract:Sixty-five Chrysanthemum cultivars were analyzed.The genetic diversity.Six pairs of primer combinations generated 244 bands in total,in which 178 bands were polymorphic.The average percentage of polymorphic bands was 72.95%.Cluster analyses by UPGMA showed that there were close genetic relationships among cultivars of the same petal type.Their origin was correlated with the analysis cluster results at a certain level.But the flower color had no clear pertinence with the cluster results.
Keywords:Chrysanthemum  Genetic diversity  Phylogenetic relationship  AFLP
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