海洋木栖真菌抗肿瘤活性及其营养条件的影响

对从厦门海沧及漳州浮宫的红树林生态区分离到的177株海洋木栖真菌用MTT法进行抗肿瘤活性的测定，获得具有抗肿瘤活性的菌株共44株，占总受试菌株的24．9％，其中对KB瘸株有抑制作用的有12株．对Raji瘤株有抑制作用的菌株有43株，对这两种肿瘤细胞均有抑制作用的菌株有11株．并且考察了其中6株海洋木栖真菌在纤维素、羧甲基纤维素钠、淀粉和蔗糖为碳源的生物量和抗肿瘤活性。结果表明海洋木栖真菌在不同营养条件下的菌体生物量及代谢产物的抗肿瘤活性均存在差异，部分菌株在纤维素为碳源时，有最高的生物量和抗肿瘤活性，PDB培养基不宜作为抗肿瘤活性测定或生产发酵的基质．

Study of Antitumor Activity and Cultivate Condition of Lignicolous Marine Fungi

Marine microorganisms have become an important source of pharmacologically active metabolites.More specially, fungi from marine environment have shown great potential.In this investigation,we focused on screening lignicolous marine fungi with antitumor activity,the data of which can be given to the future research of bioactive metabolizes as references.177 strains of lignicolous marine fungi were isolated from mangrove debris and other submerged wood panels which were collected from the intertidal zone around Xiamen.By using two cell line panels and MTT assay,24.9% of all strains have the antitumor activity.Among them,12 strains show antitumor activity against KB cell line,43 against Raji cell line and 11 against both KB cell and Raji cell line and 15 strains can inhabit the growth of KB or Raji cell line (ID_(50)>60).Meanwhile,6 strains were picked out for the research of cultivation condition in different media.The results show that distinctions exist not only in the products,but the antitumor activity.Some strains growing in cellulose medium have the highest biomass and antitumor activity.In addition,the results indicate that PDB is not a suitable medium for antitumor activity screening.