苔藓分子生物学的一些进展

本文简述了近年来苔藓分子生物学的突破性进展。 1995年以前 ,苔藓系统学中尚无DNA序列数据 ,但到 1999年产生了 2 5 0个分类单位的 10 0 0个DNA序列 ,并分析了它们的系统发生信息。在分子遗传学研究中 ,小立碗藓Physcomitrellapatens、葫芦藓Funariahygrometrica、土生墙藓Tortularuralis和角齿藓Cer atodonpurpureus正在发展成有力的工具。表达序列靶 (ESTs)和外源基因的表达已成功地用于这些苔藓植物。同源重组在酵母和小白鼠细胞中已经确定 ,然而至今尚无任何植物模型体系。 1997年Schaefer和Zryd证明 ,小立碗藓具有有效的同源重组能力 ,至今在所有植物中是独特的 ,这在分析植物基因功能中将显示是十分有用的

Some progress in molecular biology of bryophytes

This paper reviewed recent break throgh in molecular biology of bryophytes. Until 1995, there was no DNA sequence data entrying into bryophyte systematics, however, in 1999, around 1000 sequences, representing may be 250 taxa, were generated on analyzed for the phylogenetic signal they carry. For the study of molecular genetics Physcomitrella patens, Funaria hygrometrica, Ceratodon purpureus, and Tortula ruralis have been developing as powerful experimental tools. The techniques of expressed sequence tags, (ESTs) and the expression of foreign genes have been successs fully used in these tools. Homologous recombination is a well-established tool in both yeast and murine cells that until recently was unknown in any plant model system. In 1997, Schaefer and Zryd proved that the ability to perform efficient homologous recombination in Physcomitrella patens is at present unique amongst all plants and represents a powerful technique for the functional analysis of plant genes.