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脯氨酸和硝酸银对甜玉米幼胚愈伤组织诱导与分化的影响
引用本文:窦秉德,张永霞,陈莉.脯氨酸和硝酸银对甜玉米幼胚愈伤组织诱导与分化的影响[J].淮阴师范学院学报(自然科学版),2006,5(3):233-237.
作者姓名:窦秉德  张永霞  陈莉
作者单位:淮阴师范学院,生物系,江苏,淮安,223300;新疆农业大学,农学院,新疆,乌鲁木齐,830052;淮阴师范学院,生物系,江苏,淮安,223300
基金项目:江苏省高校高新技术产业发展项目
摘    要:以甜玉米TD08、TD10的幼胚为外植体,利用不同培养基对其幼胚进行愈伤组织诱导,着重探讨脯氨酸对玉米愈伤组织诱导的影响,同时检验硝酸银是否能替代脯氨酸在玉米愈伤组织诱导中所起的作用.结果表明,脯氨酸有利于玉米愈伤组织诱导且愈伤质量较好;硝酸银不能替代脯氨酸在玉米愈伤组织诱导及分化中所起的作用.

关 键 词:甜玉米  幼胚  培养基  愈伤组织  诱导率
文章编号:1671-6876(2006)03-0233-05
收稿时间:2006-05-06
修稿时间:2006年5月6日

Influence of L-Pro and AgNO3 on Callus Inducing and Differentiation in Sweet Corn
DOU Bing-de,ZHANG Yong-xia,CHEN Li.Influence of L-Pro and AgNO3 on Callus Inducing and Differentiation in Sweet Corn[J].Journal of Huaiyin Teachers College(Natrual Science Edition),2006,5(3):233-237.
Authors:DOU Bing-de  ZHANG Yong-xia  CHEN Li
Abstract:To test the function of different ingredient in cultural medium,the young embryos of sweet corn TD08 and TD10 were used as explant in this cultural experiment, mainly concerning the L-Pro influence on the callus inducing and differentiation.The results indicated that L-Pro was fit to add in cultural medium to get a high rate and good quality of callus,AgNO_3 was not suitable to substitute the function of L-Pro in callus inducing and differentiation.
Keywords:sweet corn  immature embryo  callus  inducing frequency  differentiation
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