| 产冷活性淀粉酶嗜冷菌的分离和纯化 |
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| 引用本文： | 黄秋婵 何彬. 产冷活性淀粉酶嗜冷菌的分离和纯化[J]. 广西右江民族师专学报, 2006, 19(3): 52-56 |
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| 作者姓名： | 黄秋婵 何彬 |
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| 作者单位： | [1]南宁师范高等专科学校化学与生命科学系,广西龙州532400 [2]北京市景山中学,北京100087 |
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| 摘 要： | 文章主要对产冷活性淀粉酶的冷适应微生物进行分离和纯化。通过对菌株的分离和提取基因组DNA进行PCR扩增得到其16S rRNA基因后连接于T-载体再转入大肠杆菌JM109中，筛选阳性克隆转化子进行培养，最后提取质粒进行琼脂糖凝胶电泳检测。
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| 关 键 词： | 冷活性 淀粉酶 嗜冷菌 |
| 文章编号： | 1008-8113（2006）03-0052-05 |
| 收稿时间： | 2006-02-21 |
Isolating and Purifying Psychrophiles of Cold-active Amylase |
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| HAUNG Qiu-chan , HE Bin. Isolating and Purifying Psychrophiles of Cold-active Amylase[J]. Journal of Youjiang Teachers College For Nationalities Guangxi, 2006, 19(3): 52-56 |
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| Authors: | HAUNG Qiu-chan HE Bin |
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| Affiliation: | 1. Department of Chemistry and Life Science, Nanning Teachers College, Longzhou532400. China 2. Beijing Jingshan Middle School, Beijing 100087, China |
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| Abstract: | This paper states how to isolate and purify the cold-adapted organism of the cold-active amylase. The genomic DNA isolated, and the 16S rDNA is amplified , and then the PCR product is purified and inserted to the T-vector and then transformed to JM109; recombinant clones can be identified by color screen. At last, the recombinant vector is isolated and sent to the biological corporation for the electrophoresis. |
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| Keywords: | cold active amylase psychrophiles |
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