An enzyme immunoassay for mouse epidermal growth factor utilizing a liquid phase double-antibody system |
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| Authors: | M Kashimata M Hiramatsu N Minami Jr N Minami |
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| Institution: | (1) Department of Dental Pharmacology, Josai Dental University, 350-02 Sakado, Saitama, Japan |
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| Abstract: | Summary An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF- -galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. |
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| Keywords: | Epidermal growth factor enzyme immunossay mouse |
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