| ELISA定量测定单克隆抗体方法改进 |
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| 引用本文： | 陈瑞川,卢方,卢玉英.ELISA定量测定单克隆抗体方法改进[J].厦门大学学报(自然科学版),1991(4). |
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| 作者姓名： | 陈瑞川 卢方 卢玉英 |
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| 作者单位： | 厦门大学抗癌研究中心
(陈瑞川，卢方)，厦门大学抗癌研究中心(卢玉英) |
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| 摘 要： | 本文介绍了以亲和层析纯化的免抗鼠IgG类抗体及其酶标物作为捕获及检测抗体进行鼠杂交瘤培养上清及腹水中IgG类单克隆抗体的ELISA定量测定方法。结果表明:小牛血清(BS)、免血清(RS)、马血滑(HS)、人血清(HuS)及非抗体产生细胞的培养上清对本项εLISA实验无干扰,使测定的结果较可靠,测定的鼠IgG各亚类的标准曲线的线性范围均在5～100ng/ml之间,因此这个快速、可靠的方法可为鼠IgG类单克隆抗体的定量测定提供一种实用的手段。
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| 关 键 词： | ELISA 定量测定 单克隆抗体 |
The Quantitation of IgG Monoclonal Antibodies in Hybridoma Culture Supernatants and Ascites by ELISA |
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| Chen Ruichuan Lu Fang Lu Yuying.The Quantitation of IgG Monoclonal Antibodies in Hybridoma Culture Supernatants and Ascites by ELISA[J].Journal of Xiamen University(Natural Science),1991(4). |
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| Authors: | Chen Ruichuan Lu Fang Lu Yuying |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) has been investigated forqnantitating IgG monoclonal antibodies by using affinity-purified rabbit antimouse IgG antibody and its HRP conjugate for capture and detection. The results proved that the bovin serum, horse serum, rabbit serum, human serum and non-antibody producing cell culture super natants did not interfere with the ELISA. Therefore, the results were reliable . The linear ranges of standard curves of all mouse IgG subclasses were in the 5-100 ng/ml. This rapid and reliable assay may allow practically applicable in many situations in which quantiting murine monoclonal antibodies is needed. |
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| Keywords: | ELISA Quantitation McAb |
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