左心辅助泵内皮细胞化的实验研究

培养了猪主动脉血管内皮细胞，用预衬细胞外基质结合作用ｂＦＧＦ的方法实现静置条件下ＰＵ材料的内皮细胞化，并观察内皮化材料在ＬＶＡＰ泵腔内模拟流体条件下内皮细胞的贴壁率和丢失率以及细胞形态变化。结果显示：用预衬基质结合使用ｂＦＧＦ的方法，静置条件下可以实现ＰＵ材料内皮化，镜下观察细胞形态良好，在ＬＶＡＰ泵腔内模拟流体条件下中１和２ｈ后，细胞贴壁率７４．４２％和５９．７０％，丢失率分别为２５．５８％和４

A Study on the Endothelialization Mechanism of Left Ventricular Assist Pump

The cultural model of poreine aortic endothelial cell(EC) was set up.By combined use of basic fibroblast growth factor (bFGF) and extracellular matrix (ECM),the endothelialization on PU under static culture was accomplished and the attachment rate as well as the morphological changes of EC under flowing shear stress condition were observed when LVAP was working in nomal blood circulatory pressure (12kPa).The results revealed that EC treated with bFGF grew very well on the PU material precoated with fibronectin or collagen IV.Under shear stress condition for 1 or 2 hours,the percentage of cell attachment attained 74.42% and 59.7%.The general morphological changes of the cells were not obvious under the scanning microscope,slight contraction and cell death or loss were found.This work was done to find out the condition and method of experiment for the accomplishment of endothelialization under the flowing shear stress in the cavity of LVAP.