Spacer length variation in rice 5S rRNA genes revealed by polymerase chain reaction |
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Authors: | Yi Qingming Liu Guoping |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | Polymerase chain reaction (PCR) was used to amplify 5S rRNA spacer from wild rice (Oryza rufipogon and O. nivara) and cultivated
rice (indica and japonica varieties of O. sativa L). The results show that there is spacer length variation within and between
species, and the typical indica and japonica varieties have their unique banding patterns of amplified 5S rRNA spacers, whereas
intermediate showed no specific amplification profile of spacer regions. The 5S rRNA genes in intermediate are either identical
with that of indica variety or that of japonica variety. These data suggest that the spacer length polymorphisms can be used
to distinguish between closely ralated species and subspecies.
Supported by the National Natural Science Foundation of China
Yi Qingming, born in Apr. 1938, Professor |
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Keywords: | Rice 5S rRNA genes PCR |
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