| NCED3基因雌二醇诱导表达对ABA合成酶基因和代谢酶基因表达的影响 |
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| 引用本文： | 魏开发,贾文锁,林子英,蔡月琴.NCED3基因雌二醇诱导表达对ABA合成酶基因和代谢酶基因表达的影响[J].湖北民族学院学报(哲学社会科学版),2009,27(1). |
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| 作者姓名： | 魏开发 贾文锁 林子英 蔡月琴 |
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| 作者单位： | 魏开发,林子英,蔡月琴,WEI Kai-fa,LIN Zi-ying,CAI Yue-qin(漳州师范学院生物科学与技术系,福建,漳州,363000);贾文锁,JIA Wen-suo(中国农业大学农学与生物技术学院,北京,100094)
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金，福建省自然科学基金 |
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| 摘 要： | 胞内ABA信号系统是否存在正反馈调节,一直存在争论.借助于雌二醇诱导的基因表达体系,对ABA合成关键酶基因NCED3超表达,结果发现,由于NCED3限速酶基因的高表达提高了内源ABA信号水平,进而上调了ABA合成酶基因ZEP、AA03等的表达水平,-gitt~了代谢酶基因CYPT07A3的表达.研究显示内源ABA信号水平存在正负两种反馈调节机制,植物特定生理过程中ABA信号池的高低是两种调节的协同结果.
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| 关 键 词： | ABA合成酶基因 雌二醇 ABA信号系统 反馈调节 |
Effect of NCED3 Gene Estradiol-induced Expression on ABA Biosynthetic Genes and Metabolic Enzyme Gene Expression |
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| WEI Kai-fa,JIA Wen-suo,LIN Zi-ying,CAI Yue-qin.Effect of NCED3 Gene Estradiol-induced Expression on ABA Biosynthetic Genes and Metabolic Enzyme Gene Expression[J].Journal of Hubei Institute for Nationalities(Natural Sciences),2009,27(1). |
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| Authors: | WEI Kai-fa JIA Wen-suo LIN Zi-ying CAI Yue-qin |
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| Institution: | 1.Department of Biological Sciences and Biotechnology;Zhangzhou Normal College;Zhangzhou 363000;China;2.College of Agronomy and Biotechnology;China Agricultural University;Beijing 100094;China |
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| Abstract: | There has been a heated debate on the existence of positive feedback regulation in endogenous ABA signaling system.By estradiol-induced gene expression system,the over-expression of key enzyme NCED3 gene in ABA synthesis is given.It is found the over-expression of key enzyme NCED3 gene has increased the level of endogenous ABA,thus enchanced the ABA synthase gene ZEP,AAO3 expression level,and also activated CYP707A3 metabolic enzyme gene expression.Studies have shown that endogenous ABA signaling level is c... |
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| Keywords: | ABA biosynthetic genes estradiol ABA signal system feedback regulation |
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