| 基于区域信息的悬浮细胞识别与计数 |
| |
| 引用本文： | 黄红梅,王娜,彭青玉.基于区域信息的悬浮细胞识别与计数[J].暨南大学学报,2007,28(5):466-469. |
| |
| 作者姓名： | 黄红梅 王娜 彭青玉 |
| |
| 作者单位： | 暨南大学计算机科学系,广东,广州,510632;暨南大学计算机科学系,广东,广州,510632;华南师范大学增城学院计算机科学系,广东,广州,511363 |
| |
| 摘 要： | 悬浮细胞的培养过程需要对活细胞计数.针对人工计数方法存在的缺陷,利用图像分析技术对拍摄的细胞图片进行处理,实现活细胞自动识别及计数.要实现细胞识别,首先要对图像进行正确分割.对于前景和背景对比度较小且细胞很稀疏的图像,采用背景拟合设定阈值进行分割;针对前景和背景对比度较大的图像,采用一种基于局部区域直方图的方法进行分割.对分割后的图像进行链码跟踪并测量细胞参数,最终实现细胞识别及计数.实验结果表明上述方法能快速有效地计算出活细胞个数.
|
| 关 键 词： | 悬浮细胞 拟合 直方图 链码 |
| 文章编号： | 1000-9965（2007）05-0466-04 |
| 修稿时间： | 2007-04-16 |
Recognition and counting of suspension cells based on regional information |
| |
| HUANG Hong-mei,WANG Na,PENG Qing-yu.Recognition and counting of suspension cells based on regional information[J].Journal of Jinan University(Natural Science & Medicine Edition),2007,28(5):466-469. |
| |
| Authors: | HUANG Hong-mei WANG Na PENG Qing-yu |
| |
| Institution: | 1. Department of Computer Science, Jinan University, Guangzhou 510632, China; 2. Department of Computer Science, College of Zengcheng, South China Normal University, Guangzhou 511363,China |
| |
| Abstract: | It is needed to count live cells after suspension ceils being cultured. To solve problems caused by counting ceils manually, live cells are recognized and counted automatically by image-analyzing technique. Two methods are used to segment two different kinds of ceil images respectively. One method is based on background fitting to the gray of original cell image and the other on a local histogram. Then the parameters of every cell can be obtained by tracking a segmented image by chain code. With these parameters, all cells in a cell image are tracked and counted quickly and efficiently. |
| |
| Keywords: | suspension cell fitting histogram chain code |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
