采用S/W/O/W法制备BSA-PLG微球

以生物可降解材料PLG607为载体, 采用S/W/O/W溶剂挥发法制备牛血清白蛋白(BSA)微球, 对其性质进行表征, 并考察了处方中不同添加剂对BSA-PLG微球粒径、 包封率和体外释放的影响. 结果表明, 采用S/W/O/W法所得微球粒径为40～70 μm, 表面光滑圆整. 加入添加剂（m(羟丙基 β 环糊精) ∶m(牛血清白蛋白)=1 ∶1）后包封率可以从27.9%提高到46.3%, 加入添加剂羟丙基-β-环糊精和羟基磷灰石制备蛋白微球, 能减小突释, 提高蛋白的包封率, 改善体外释放行为, 达到均匀释放.

Preparation of BSA-PLG Microspheres by S/W/O/W Method

The BSA PLG microspheres were prepared by S/W/O/W emulsionsolvent evaporation method with biodegradable material PLG607 as the carrier. Drug encapsulation capacity, morphology, particle size characteristics, as well as in vitro release profiles of the microspheres were discussed. The results show that the microspheres were spherical and smooth and the mean size of the microspheres was 40~70 μm, with a mono dispersibility. The in vitro release profiles of the microspheres produced from different methods were significantly distinct. With the additives of hydroxypropyl β cyclodextrin (HPB) and hydroxyapatite (HAP), the encapsulation of microspheres increased from 27.9% to 46.3%. Therefore, using S/W/O/W method and adding additives HAP and HPB in theformulation could decrease the initial burst release of drugs from the microspheres and increase the encapsulation capacity of the BSA PLG microspheres, the in vitro release profiles of which were fitted into near zero order releasekinetics.