| 凯氏定氮法测定啤酒酵母碱溶性多糖中蛋白质的质量分数 |
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| 引用本文： | 于文阁,张丽霞,张莲姬. 凯氏定氮法测定啤酒酵母碱溶性多糖中蛋白质的质量分数[J]. 高师理科学刊, 2008, 28(4) |
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| 作者姓名： | 于文阁 张丽霞 张莲姬 |
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| 作者单位： | 1. 大庆师范学院,生命科学系,黑龙江,大庆,163712
2. 延边大学,农学院,吉林,龙井,133400 |
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| 摘 要： | 从啤酒酵母中提取出碱溶性粗多糖ASP,经乙醇分级、savag法脱蛋白以及分子筛层析进一步纯化得ASP2和ASP3,采用半微量凯氏定氮法,通过消化、蒸馏、滴定后计算其蛋白质质量分数,ASP,ASP2,ASP3分别为15.41%,3.17%和2.89%.
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| 关 键 词： | 凯氏定氮法 蛋白质 啤酒酵母碱溶性多糖 |
Determination of protein content in a alkaline-soluble polysaccharide of Beer Yeast by Kjeldahl method |
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| YU Wen-ge,ZHANG Li-xia,ZHANG Lian-ji. Determination of protein content in a alkaline-soluble polysaccharide of Beer Yeast by Kjeldahl method[J]. Journal of Science of Teachers＇College and University, 2008, 28(4) |
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| Authors: | YU Wen-ge ZHANG Li-xia ZHANG Lian-ji |
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| Abstract: | A alkaline-soluble crude polysaccharide ASP isolated from the caput-mdusa of S.cerevisiae has been fractionated.The matter was been deproteinizationed by the evage,alcohol fractional precipitation and purified by column chromatography.So ASP2 and ASP3 was abtained by purification further.Which were digested and distilled and titrated with hydrochloric acid standard solution to give the protein content of ASP,ASP2 and ASP3 were determined 15.41%,3.17% and 2.89 % respectively. |
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| Keywords: | Kjeldahl method protein alkaline-soluble polysaccharide of Beer Yeast |
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