红螯光壳螯虾Hsp70基因的特征及其在热应激下的表达

从NCBI数据库中获得红螯光壳螯虾CqHsp70的cDNA全长2231 bp，可编码643个氨基酸，其中4—598aa为HSP70结构域。实时定量PCR结果表明，CqHsp70基因在红螯光壳螯虾的鳃、肝胰腺、血液中均有表达。在高温（34 ℃）应激过程中，红螯光壳螯虾CqHsp70基因在三个组织中的表达量呈先升高后降低的模式，在12 h时表达量最高，与对照组存在显著性差异。这一结果说明高温应激后的12 h是红螯光壳螯虾调控热应激的关键点，在夏天高温季节的养殖过程中应特别加以注意。

Characterization of Hsp70 Gene and Its Expression Under Heat Stress in Cherax quadricarinatus

In this study,a 2231 bp full length cDNA of CqHsp70 was obtained from NCBI.It encodes 643 amino acids containing an HSP70 domain (4-598aa).Quantitative real-time PCR results showed that CqHsp70 gene was expressed in the gill,hepatopancreas,and haemocytes.In the stage of high-temperature(34 ℃)stress,the expression level of the CqHsp70 gene in the three tissues all increased first and then returned to normal,with the highest expression at 12 h.The results showed that 12 hours after high-temperature stress was the key point of regulatory response,and special attention should be paid during the process of red claw crayfish culture in the hot summer.