| 钝顶螺旋藻分离纯化SOD的中试生产 |
| |
| 引用本文： | 谢婷玉,张亚旗,卢珍华,苏国成,张铃玉.钝顶螺旋藻分离纯化SOD的中试生产[J].集美大学学报(自然科学版),2021,26(4):314-319. |
| |
| 作者姓名： | 谢婷玉 张亚旗 卢珍华 苏国成 张铃玉 |
| |
| 作者单位： | （集美大学海洋食品与生物工程学院，福建 厦门 361021） |
| |
| 摘 要： | 以钝顶螺旋藻(Spirulina platensis)干粉为实验原料,经过超滤、两步盐析、DEAE离子交换色谱柱层析和SuperdexTM200凝胶过滤柱层析,逐步纯化超氧化物歧化酶(superoxide dismutase,SOD).结果表明,在90 L粗藻液的中试放大体系中,所得酶液的平均比活力为4131 U/mg...
|
| 关 键 词： | 钝顶螺旋藻 超氧化物歧化酶 分离 纯化 中试生产 |
Pilot Scale Production of Superoxide Dismutase in Spirulina platensis |
| |
| XIE Tingyu,ZHANG Yaqi,LU Zhenhua,SU Guocheng,ZHANG Lingyu.Pilot Scale Production of Superoxide Dismutase in Spirulina platensis[J].the Editorial Board of Jimei University(Natural Science),2021,26(4):314-319. |
| |
| Authors: | XIE Tingyu ZHANG Yaqi LU Zhenhua SU Guocheng ZHANG Lingyu |
| |
| Institution: | （College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021,China） |
| |
| Abstract: | In this study,superoxide dismutase (SOD) was isolated from Spirulina platensis,and then sequentially purified through ultrafiltration,two-step salting-out method,DEAE ion exchange chromatography and SuperdexTM200 gel filtration chromatography.The results showed that the average specific of the enzyme was 4 131 U/mg,the purification multiple was 238.96 and the extraction recovery of this protein was 8.3% in a pilot scale system of 90 L crude algal extract.The result of SDS-PAGE analysis demonstrated that molecular mass of SOD was 18.4 ku.Repeat experiments showed similar results.In addition,the SOD activity of three repeated experiments was all greater than 4 000 U/mg. |
| |
| Keywords: | Spirulina platensis superoxide dismutase separation purification pilot scale production |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《集美大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《集美大学学报(自然科学版)》下载免费的PDF全文 |
|
