Solubilization and characterization of a ouabain-sensitive protein from transverse tubule membranejunctional sarcoplasmic reticulum complexes (TTM-JSR) in cat cardiac muscle |
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| Authors: | S Fujino K Satoh T Bando T Kurokawa T Nakai K Takashima M Fujino |
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| Institution: | (1) Department of Pharmacology, Hokkaido Institute of Pharmaceutical Sciences, 047-02 Otaru, (Japan);(2) Department of Physiology, Boei Medical College, Tokolozawa, Saitama, (Japan) |
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| Abstract: | Summary A new glycoprotein of 31,500 dalton, which has a high affinity for ouabain, and is independent of (Na+–K+)-ATPase, was solubilized from transverse tubule membrane and junctional sarcoplasmic reticulum complexes (TTM-JSR) of cat cardiac muscle. This protein could be extracted only in small amounts from sarcolemmaplasma membrane (SLM-PL) fragments, suggesting that it indeed originates from the TTM-JSR. |
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| Keywords: | Ouabain-sensitive protein solubilization characterization TTM-JSR cat heart |
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