一种可用于条件性位置偏爱实验的疼痛大鼠模型

目的:建立大鼠背部切口疼痛模型,以条件性位置偏爱实验(CPP)观察大鼠对止痛药对乙酰氨基酚作用的主观反应。方法:在大鼠右下背部定位作手术切口并缝合后,以对乙酰氨基酚100 mg/kg伴药适应4次,每次间隔12 h,观察适应前后大鼠CPP值的变化。结果:切口痛大鼠在伴药适应后对伴药箱CPP值显著增加,而非手术用药大鼠及未用药对照组大鼠的CPP值变化无显著意义。结论:背部切口痛大鼠模型可以用于CPP实验,有助于观察大鼠对镇痛药的奖赏效应以及实验者评估动物对疼痛的主观不愉快感受。

A Rat Pain Model for Conditioned Place Preference Experiment

Objective:Establishing an back incision pain rat model to observe the subjective re action of rats to acetaminophen with conditioned place preference(CPP)experiment.Methods: Made a skin cut then suture at the right lower back of rat,then used acetaminophen 100 mg/kg and observed CPP values for 4 times at 12 hours' interval;comparing the difference between preCPP and postCPP.Results:The postCPP values of incision pain rats were significantly increased compared with preCPP(P < 0.05),while those of sham rats and control rats were not when compared with their preCPP.Conclusion:This rat back incision pain model is suitable for observing the reward ef fects of pain relieve agents with CPP experiment,and will be helpful to assess the subjective un pleasantness of animals to pain.