| 考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量 |
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| 引用本文： | 罗群.考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量[J].成都大学学报(自然科学版),2014(2):125-126,129. |
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| 作者姓名： | 罗群 |
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| 作者单位： | 成都师范学院教育科学学院,四川成都611130 |
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| 基金项目： | 四川省教育厅自然科学基金（11ZB076）资助项目. |
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| 摘 要： | 采用考马斯亮蓝G-250染色法对菜籽粕中可溶性蛋白质含量进行测定,在波长595 nm处测定其吸光度.实验表明,可溶性蛋白质含量在10~100μg/mL之间呈现良好的线性关系,通过此法测得菜籽粕中可溶性蛋白的平均含量为5.30%.方法简便快速、灵敏度高、重现性好,是测定菜籽粕中可溶性蛋白质的一种有效方法.
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| 关 键 词： | 菜籽粕 可溶性蛋白 考马斯亮蓝法 |
Rapid Determination of Soluble Protein Content in Rapeseed Meal By Coomassie Brilliant Blue Method |
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| Institution: | LUO Qua (School cf Education Sciences, Chengdu Normal University, Chongdu 611130, Ofina) |
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| Abstract: | We use the dying method of Coomassie brilliant blue G - 250 to determine the protein content in rapeseed meal, and measure the absorbency at the wavelength 595 nm. The experimental results show that the protein content presents a good linear relationship in the range of 10 ~ 100μg/mL. This method has advantages of simplicity, rapidity, high sensitivity and good reproducibility, which is an effective method to determine the soluble protein content in rapeseed meal. The average content of soluble protein in the rapeseed meal is 5.30% by this method. |
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| Keywords: | rapeseed meal soluble protein Coomassie brilliant blue method |
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