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| HPLC测定贵州产知母药材中菝葜皂苷元的含量 | |
| 引用本文： | 彭全材,胡继伟,杨占南,周秀玲,夏品华,黄先飞.HPLC测定贵州产知母药材中菝葜皂苷元的含量[J].贵州师范大学学报(自然科学版),2007,25(3):98-101. |
| 作者姓名： | 彭全材 胡继伟 杨占南 周秀玲 夏品华 黄先飞 |
| 作者单位： | 1. 贵州师范大学,贵州省山地环境重点实验室,贵州,贵阳,550001;贵州师范大学,生物技术与工程学院,贵州,贵阳,550001 2. 贵州师范大学,贵州省山地环境重点实验室,贵州,贵阳,550001 3. 江西宜春学院,化生学院,江西,宜春,336000 |
| 摘 要： | 实验采用乙醇超声提取样品,HPLC梯度洗脱。菝葜皂苷的理论塔板数为5628,分离度为1.42,拖尾因子为1.01。回归方程为Y=5×106X+610 06,r=0.9990.在0.4~2.0mg/mL之间的线性关系良好。日内和日间精密度的RSD均小于2.0%,重复性试验的RSD为0.6%(n=5),最低检测限为1.0μg/mL,加样回收率为96.5%、94.3%、97.2%,RSD(n=5)分别为2.7%、3.1%、2.6%。 |
| 关 键 词： | HPLC-ELSD 菝葜皂苷 知母药材 |
| 文章编号： | 1004-5570（2007）03-0098-04 |
| 收稿时间： | 2007-04-18 |
| 修稿时间： | 2007-04-18 |
Determination of Sarsasapogenin in Anemarrhenaasphodeloides Bge from Guizhou by high-performance liquid chromatography |
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| PENG Quan-cai,HU Ji-wei,YANG Zhan-nan,ZHOU Xiu-ling,XIA Ping-hua,HUANG Xian-fei.Determination of Sarsasapogenin in Anemarrhenaasphodeloides Bge from Guizhou by high-performance liquid chromatography[J].Journal of Guizhou Normal University(Natural Sciences),2007,25(3):98-101. | |
| Authors: | PENG Quan-cai HU Ji-wei YANG Zhan-nan ZHOU Xiu-ling XIA Ping-hua HUANG Xian-fei |
| Abstract: | The medicinal plant was extracted with ethanol and the extraction was repeated for three times.The theoretical plate number of astragaloside Ⅳ was 5628,the resolution 1.42 and the tailing factor 1.01.The calibration curve was linear in the range of 0.4～2.0mg/mL,Y= 5×106X +61006,R = 0.9990.The intra-day and inter-day precisions(RSD) were all less than 2.0%,the RSD of recurrence was 0.6%(n=5).The limit of detection was 1.0 μg/ml,the recovery 96.5%,94.3%,97.2%,and RSD 2.7%,3.1%,2.6%(n=5) respectively for Sarsasapogenin. |
| Keywords: | high-performance liquid chromatography with evaporative light-scattering detection Sarsasapogenin Anemarrhenaasphodeloides Bge |
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