Isolation of tomato proteinase inhibitor II gene and the function of its intron |
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Authors: | Xianzhi Xie Naihu Wu |
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Institution: | (1) Institute of Developmental Biology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | The genomic DNA sequence of tomato proteinase inhibitor II gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length
of 109 bp, owns typical structures of GT/AG dinucleotides at both ends and high content of AT base pairs which accounts for
80.7% of the total nucleotides. As shown by recombination experiment, the TPI sequence could efficiently promote the expression
of the reporter gene gusA and this effect was independent of the position and orientation of the intron, thus showing its role as an enhancer. Such
experiments as gel retardation assays, GUS histochemical staining and GUS fluorometric assays further demonstrated that TPI
sequence maybe has promoter-like activity. |
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Keywords: | tomato proteinase inhibitor II gene intron enhancer |
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