款冬组织培养与快繁技术的研究

以款冬根状茎为外植体，将其接种于诱导培养基上，培养30d后形成愈伤组织，增殖培养后分割，在分化培养基上诱导茎、叶、根的分化形成试管苗；带节根状茎段在分化培养基上培养，由茎节处萌发新枝，20-30d后形成丛生苗，分割后培养可长成具有不定根的试管苗。通过诱导愈伤组织和丛生苗两条途径均可快速繁殖款冬的试管苗，试管苗经过“炼苗”后移栽，生长良好，这为人工栽培解决款冬“种苗”及其开发利用有重要的实用价值。

Studies on tissue culture and rapid propagation of Tussilago farara. L.

Tussilago farfara. L. is a commonly wild medical plant. Therhizome of Tussilago farfara. L. as explant was inoculated into induction medium and produced callus after 30 days.The proliferated calli were cut into many small parts and put into fresh differentiation medium to induce the formation of stalks,leaves and roots for producing seedling.The rhizome with nod when cultured on medium formed sprouts from nod and eventually clustered plantlets 20 to 30 days later. Further cultivation of clustered plantlets after cutting produced adventitious roots. Tussilago farfara. L. can rapidly propagated by either induced callus or clustered plantlets. The regenerated seedlings after acclimatization grew well after they were transplanted. The success in Tussilago farfara. L. tissue culture can provide seedling for artificial cultivation form. This is of great value to the exploitation and utilization of Tussilago farfara. L..