益生菌制剂对虹鳟血清生化指标和免疫功能的影响

本文研究了饲料中添加益生菌制剂(丁酸梭菌、凝结芽孢杆菌)对虹鳟血清生化指标、非特异性免疫功能以及抗病力的影响，结果表明：不同单一益生菌制剂、复合益生菌制剂均能提高虹鳟血清总蛋白及葡萄糖含量(P0.05)，降低胆固醇、甘油三酯含量(P0.05)；复合益生菌制剂可显著提高虹鳟血清ACP、T-AOC、CAT、SOD活性，降低MDA含量，且凝结芽孢杆菌T2比丁酸梭菌T1效果好(P0.05)；复合益生菌制剂显著提高了虹鳟血清补体C3含量，间接提高机体非特异性免疫功能；益生菌制剂提高了虹鳟机体的抗病力，其中复合益生菌制剂组累积死亡率最低(23.1%，P0.05)，免疫保护率最高（66.6%，P0.05). 综上所述，丁酸梭菌和凝结芽孢杆菌均对虹鳟机体免疫功能具有促进作用.

Effects of dietary probiotics on serum biochemistry and immunity of Rainbow Trout,Oncorhynchus mykiss

The study was conducted to test the effects of dietary probiotics (Clostridium butyricum, Bacillus coagulans) on biochemistry, non-specific immunity and disease resistance in the serum of Rainbow Trout, Oncorhynchus mykiss. The study produced the following results. First, compared to the control treatment T0, there was a higher increase of the contents of total protein and glucose in the serum of O. mykiss fed with signal and multiple probiotics (P0.05), and meanwhile there was a decrease of the contents of total-cholesterol and triglycerides (P0.05). Second, the combination of multiple probiotics, Clostridium butyricum and Bacillus coagulans, was thought to be even more beneficial than a single probiotic. The activities of acid phosphatase (ACP), total antioxidant capacity (T-AOC), catalase (CAT), superoxide dismutase (SOD) in treatment T3 were significantly improved (P0.05), and the content of malondialdehyde (MDA) was significantly reduced, with treatment T2 with Bacillus coagulans having a better effect than treatment T1 with Clostridium butyricum. The content of Complement C3 in treatment T3 with mixed probiotics was significantly increased. These could enhance the non-specific immunity of O. mykiss. Third, supplementation of different probiotics could decrease the cumulative mortality of O. mykiss and improve the relative percent survival (RPS). The lowest cumulative mortality and the highest RPS of O. mykiss were found in treatment T3, they were 23.1% and 66.6% respectively. It can be concluded that the supplementation of Clostridium butyricum and Bacillus coagulans in diets can enhance the immunity of O. mykiss.