RAPD法探究百草枯对HepG2细胞基因组稳定性的影响

目的:探讨百草枯(PQ)处理对HepG2细胞基因组稳定性的影响.方法:采用随机扩增多态性DNA(RAPD)标记技术,选取20种随机引物分析PQ处理24h对HepG2细胞基因组稳定性的影响.结果:引物5’-GGAAGTCGCC-3’、5’-ACGCGCATGT-3’和5’-GAATCGGCCA-3’的扩增产物在正常组与7.21、10.67、15.78、22.36和34.57mg/L的PQ处理组间有较明显差异.其中,引物5’-GGAAGTCGCC-3’的2种扩增产物(约2 200和2 600bp)在所有PQ处理组均缺失;引物5’-ACGCGCATGT-3’的2种扩增产物(约250和1 200bp)在高于7.21mg/L的PQ处理组均发生缺失,1种扩增产物(约1 500bp)在34.57mg/L的PQ处理组发生缺失,另有2种扩增产物(约400和550bp)在34.57mg/L的PQ处理组含量较低;引物5’-GAATCGGCCA-3’有2种扩增产物(约900和1 900bp)在全部PQ处理组均缺失,另2种扩增产物(约530和560bp)新出现在各PQ处理组.结论PQ处理会破坏HepG2细胞基因组的完整性和稳定性.

The effects of paraquat on genome stability in HepG2 cells by RAPD labeling technique

Objective:To explore the effects of paraquat(PQ)on genome stability in HepG2 cells.Methods:By randomly amplified polymorphic DNA(RAPD)labeling technique,the paper selected 20 kinds of random primers to analyze the effects of 24 h-PQ treatment on genome stability in HepG2 cells.Results:The amplification products of the primers 5'-GGAAGTCGCC-3',5'-ACGCGCATGT-3'and 5'-GAATCGGCCA-3'were significantly different between the normal group and 7.21,10.67,15.78,22.36,34.57 mg/L of PQ treatment groups.For the primer 5'-GGAAGTCGCC-3',two kinds of amplification products(about 2 200 and 2 600 bp respectively)were absent in all PQ treatment groups.For the primer 5'-ACGCGCATGT-3',two kinds of amplification products(about 250 and 1 200 bp respectively)were absent in greater than 7.21 mg/L of PQ treatment groups and another of amplification products(about 1 500 bp)was absent in 34.57 mg/L of PQ treatment group.In addition,two kinds of amplification products(about 400 and 550 bp respectively)were less in 34.57 mg/L of PQ treatment group than normal group.For the primer 5'-GAATCGGCCA-3',two kinds of amplification products(about 900 and 1 900 bp respectively)were absent and two kinds of new amplification products(about 530 and 560 bp respectively)were found in all PQ treatment groups.Conclusion PQ treatment could break genome integrity and stability of HepG2 cells.