一种低危害的基因组DNA和总RNA的提取方法

从生物组织里提取基因组DNA和总RNA是多数专业研究人员都需要操作的实验内容,但该实验的常规操作流程中却存在致病性微生物的感染风险和有毒有害试剂的危害.本文从选择安全的实验材料和改进低危的实验方法两个方面入手,提出了以盐藻为实验材料的基因组DNA和总RNA的新的实验提取方法,该方法有效避免了致病性微生物的感染风险,也避免了有毒、挥发性试剂苯酚、氯仿和Trizol的使用,对核酸提取实验操作的安全性提供了一定的保障,可供研究者选取实验方案时参考.

A low risk experimental method of genomic DNA and total RNA extraction

Genomic DNA and total RNA extraction are experiments for researchers of various majors. However, students and teachers encounter the risks of infectious pathogenic microorganisms and toxic chemicals. To reduce the risks, this study starts from choosing safety experimental materials and improving experimental methods. A new genomic DNA and total RNA extraction methods was developed with Duanliella salina as experimental material. The new method is a valuable reference for nucleotide extraction, which totally avoids pathogenic microorganisms, and also avoids the use of toxic, volatile chloroform, phenol and Trizol.