| 植物细胞DNA提取与PCR实验的设计及其关键参数的优选 |
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| 引用本文： | 闵运江. 植物细胞DNA提取与PCR实验的设计及其关键参数的优选[J]. 皖西学院学报, 2010, 26(2): 89-92 |
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| 作者姓名： | 闵运江 |
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| 作者单位： | 皖西学院,化学与生命科学系,安徽,六安,237012 |
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| 基金项目： | 安徽省教育厅2010年度高校省级科学研究重点项目 |
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| 摘 要： | 以草本类型的蓼科5种植物为实验材料,研究了DNA提取方法。探讨了nrDNA ITS片段的PCR扩增方法,通过正交试验法,对该实验的关键步骤中PCR体系主要成分的最适含量进行了优化。为植物细胞DNA提取和PCR扩增这一现代生物学必不可少的实验教学和相关科学研究提供方法借鉴。
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| 关 键 词： | DNA提取 PCR扩增 实验设计 正交试验 草本植物 |
The Experiment Design and Its Key Parameters Optimization of Isolation and PCR Amplification DNA Fragment from Plant Cell |
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| MIN Yun-jiang. The Experiment Design and Its Key Parameters Optimization of Isolation and PCR Amplification DNA Fragment from Plant Cell[J]. Journal of Wanxi University, 2010, 26(2): 89-92 |
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| Authors: | MIN Yun-jiang |
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| Affiliation: | MIN Yun-jiang (Department of Chemistry and Life Science,West Anhui University,Lu＇an 237012,China) |
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| Abstract: | The experiments had been designed which plant cell DNA isolation and its nrDNA ITS fragment PCR amplification,with 5 species herbaceous materials of Polygonaceae.The key step of the experiment the main components of the PCR system optimum concentration had been optimized by the orthogonal test method.The results of this experiment will provide an example to our experimental teaching and related research. |
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| Keywords: | DNA isolation PCR amplification experiment design the orthogonal test method herbaceous |
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