| 体外反应体系中APC/C活性及相关研究 |
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| 引用本文： | 高彦飞,李腾,常艳,王玉博,穆蕊,陈亮,甄诚,韩秋影,于鸣,巩伟丽,张维娜,李慧艳.体外反应体系中APC/C活性及相关研究[J].科学技术与工程,2011,11(21). |
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| 作者姓名： | 高彦飞 李腾 常艳 王玉博 穆蕊 陈亮 甄诚 韩秋影 于鸣 巩伟丽 张维娜 李慧艳 |
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| 作者单位： | 1. 国家生物医学分析中心,北京,100850
2. 军事医学科学院基础医学研究所细胞免疫学教研室,北京,100850 |
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| 基金项目： | 国家自然科学基金（批准号：30871234） |
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| 摘 要： | 为获得具有细胞周期后期促进复合物（APC/C）活性的细胞裂解液并建立APC/C体外活性检测体系，以其底物Cyclin B及Securin的降解为检测指标，分别从细胞阻滞于有丝分裂期的时间， UBCH10（APC/C的E2酶）的浓度以及裂解液浓度等几个方面研究了各种处理因素对APC/C活性的影响，并且用体外翻译底物验证了其活性及相关结论。此项工作为进一步探索APC/C相关分子事件奠定了基础。
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| 关 键 词： | 后期促进复合物 Cyclin B Securin UBCH10 活性 |
| 收稿时间： | 4/8/2011 3:05:14 PM |
| 修稿时间： | 4/8/2011 5:54:16 PM |
Activity of APC/C and its relationship with the MCC in the In vitro reaction system |
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| Gao Yanfei,Li Teng,Chang Yan,Wang Yubo,Mu Rui,Chen Liang,Zhen Cheng,Han Qiuying,Yu Ming,Gong Weili,Zhang Weina and Li Huiyan.Activity of APC/C and its relationship with the MCC in the In vitro reaction system[J].Science Technology and Engineering,2011,11(21). |
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| Authors: | Gao Yanfei Li Teng Chang Yan Wang Yubo Mu Rui Chen Liang Zhen Cheng Han Qiuying Yu Ming Gong Weili Zhang Weina and Li Huiyan |
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| Institution: | GAO Yan-fei1,LI Teng1,CHANG Yan1,WANG Yu-bo 1,MU Rui1,CHEN Liang1,ZHEN Cheng1,HAN Qiu-ying1,YU Ming2,GONG Wei-li1,ZHANG Wei-na1,LI Hui-yan1 (National Center of Biomedical Analasis1,Beijing 100850,P.R.China,Department of Molecular Immunology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences2,P.R.China) |
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| Abstract: | To analysis the activity of APC/C and its relationship with the MCC in the In vitro reaction system,through detecting the substrates' ubiquitination,several assays were done.The concentration of extracts where purified APC/C from,different substrates,and the disassociation of MCC from APC/C were analyzed.As shown in the results,the activity of APC/C purified from the higher concentration extracts was higher,the ubiquitination of Geminin was easier than the Cyclin B and during the activation of APC/C,the bin... |
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| Keywords: | APC/C Cyclin B Securin UBCH10 Activity |
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