三种鲍腹足肌胶原多肽抗氧化性研究

研究了皱纹盘鲍(Haliotis discus hannai)、黑足鲍(Haliotis iris)及其杂交鲍腹足胶原多肽的制备和性质,并采用总还原力、对O^-₂·和·OH自由基清除率和致衰小鼠灌胃胶原多肽后体内MDA、SOD和GSH-PX变化来评价3种鲍胶原多肽的抗氧化性。结果显示,皱纹盘鲍、黑足鲍及其杂交鲍的胶原蛋白分别占总蛋白含量的23.44%、30.78%和26.92%,皱纹盘鲍与杂交鲍胶原蛋白含有α1、α2、β和γ链,而黑足鲍胶原蛋白没有γ链;来自3种鲍的胶原多肽均呈现出较高的还原力、O^-₂·和·OH自由基清除能力,对O^-₂·清除率的IC₅₀值分别为3.03、2.40、1.15 g/L,对·OH清除率的IC₅₀值分别为4.12、3.58、3.70 g/L;灌胃3种胶原多肽后,致衰小鼠肝脏和血清的MDA含量均降低,SOD和GSH-PX活力均上升。三者比较,杂交鲍胶原多肽在降低MDA含量、提高GSH-PX活力的作用最强,黑足鲍胶原多肽在提高SOD活力的效果最显著。

Antioxidant activity of collagen peptides from three kinds of abalone foot muscle

Isolation and characterization of collagen from Haliotis discus hannai, Haliotis iris and their hybrid abalone were studied, the total reducing power, O^-₂· and ·OH radical scavenging rate and changes of MDA、SOD and GSH-PX of induced aging mice by gavage collagen peptides were used to assess collagen peptides antioxidant activity. The results showed that the collagen of H. discus hannai, H. iris and their hybrid accounted for 23.44%, 30.78% and 26.92% of the total protein content, respectively. H. discus hannai and the hybrid abalone contained α1, α2, β and γ chains, but H. iris did not contain γ chains. All three collagen peptides showed high level of reducing power as well as O^-₂· and ·OH radical scavenging ability. IC₅₀ values of O^-₂·radical scavenging were 3.03, 2.40 and 1.15 g/L respectively, and IC₅₀ values of ·OH radical scavenging were 4.12, 3.58 and 3.70 g/L respectively. After the three peptides were gavaged to induced aging mices, the MDA content of liver and serum decreased and SOD content and GSH-PX activity increased. The hybrid abalone was the strongest in reducing MDA content and strengthening GSH-PX activity while H. iris was the most effective in increasing SOD.