| HPLC法测定复方丹芎滴丸中葛根素的含量 |
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| 引用本文： | 贾永艳,魏超娟,田效志,李九席,樊静静. HPLC法测定复方丹芎滴丸中葛根素的含量[J]. 河南科学, 2012, 30(8): 1036-1038 |
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| 作者姓名： | 贾永艳 魏超娟 田效志 李九席 樊静静 |
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| 作者单位： | 河南中医学院药剂学院,郑州,450008 |
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| 基金项目： | 郑州市科技攻关项目（083SGYS33264-4） |
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| 摘 要： | 用高效液相色谱法测定复方丹芎滴丸中葛根素的含量,采用高效液相色谱法,色谱柱为C18柱(4.6 mm×250 mm,5μm),甲醇-水(25∶75)为流动相,流速为1.0 mL.min-1,检测波长:250 nm.结果表明,葛根素在0.076 2～1.524 mg范围内与峰面积呈良好的线性关系,R2=0.999 9,平均回收率95.6%,RSD=1.51%(n=6).说明该测定方法稳定,重现性好,结果准确可靠,可用于复方丹芎滴丸的质量控制.
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| 关 键 词： | 复方丹芎滴丸 葛根素 HPLC |
Content Determination of Puerarin in Fufangdanxiong Pills by HPLC |
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| Jia Yongyan , Wei Chaojuan , Tian Xiaozhi , Li Jiuxi , Fan Jingjing. Content Determination of Puerarin in Fufangdanxiong Pills by HPLC[J]. Henan Science, 2012, 30(8): 1036-1038 |
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| Authors: | Jia Yongyan Wei Chaojuan Tian Xiaozhi Li Jiuxi Fan Jingjing |
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| Affiliation: | (Pharmacy College, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China) |
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| Abstract: | To establish the method for the content determination of Puerarin in Fufangdanxiong pills, HPLC was adopted. The separation was performed on Agilent TC-C18 （4.6 mm×250 mm, 5 μm）, column with methanol-water （25：75） as mobile phase. The detection wavelength was 250 nm. The flow rate was 1.0 mL·min-1. Rrsuh was shown that linear range of Puerarin was 0.076 2-1.524 mg （R2=0.999 9）,with an average recovery of 95.6% （RSD=1.51%, n=6）. The method is simple, accurate and well-separated for the quality control of Fufangdanxiong pills. |
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| Keywords: | fufangdanxiong pills puerarin HPLC |
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