三叉神经慢性缩窄环术大鼠半月节射频热凝的实验研究

目的观察三叉神经慢性缩窄环术大鼠半月节射频热凝的大体及镜下改变，探讨射频热凝温度和时间与半月神经节损伤的关系。方法36只成年雄性SD大鼠，随机选取33只建立慢性缩窄性损伤三叉神经痛模型。建模成功2周后，随机分为3组：射频热凝组（R组，n=24），假手术纽（S组，n=6），热凝对照纽（C组，n=3）。经中颅窝开颅暴露半月神经节，R组行半月节穿刺热凝，根据温度、时间随机分为8个小组，每组3只，分别为：65℃120″、180″组，70℃120″、180″组，75℃120″、180″组，80℃120″、180″组。S组仅行半月节穿刺不热凝，按留针时间分为120″、180″两组；C组不行半月神经节穿刺及热凝。余3只SD大鼠作为正常对照组（N组）。R组及S组热凝或穿刺结束即取出大鼠半月神经节，C组、N组直接取出半月神经节，观察半月神经节大体变化，游标卡尺测量神经节毁损区直径，光镜下观察神经节细胞的变化。结果S组、C组与N组比较，大鼠半月神经节大体及光镜下均无明显变化。R组在65℃时大鼠半月节大体及镜下无明显损伤；70℃时开始在肉眼及镜下出现损伤；75℃、80℃时半月节则出现明显凝固区，毁损区直径测量较70℃组明显增大（P〈0．01），光镜下神经元细胞核出现不同程度的固缩改变。毁损温度越高、时间越长，损伤范围越大，程度越重。结论75℃、80℃射频热凝可导致神经节细胞明显变性，损伤程度及范围随温度升高、时间延长逐渐加重与增大。

Experimental Study of Radio Frequency on Semilunar Ganglion of Trigeminal Neuralgia Rats Following Chronic Constriction Injury

Objective To observe the histological changes of semilunar ganglions of trigeminal neuralgia rats following chronic constriction inju- ry after radiofrequency thermocoagulation （RA） ,further discuss the influences of temperature and time produced on ganglion. Methods Thirty-six male Sparague-Dawley （SD） rats, thirty-three of them had chronic constricting injury （CCI）. Week 2 after CCI, the thirty-three rats were divided into three groups randomly ： RA group （ group R, n = 24 ）, shamed operation group （ group S, n = 6 ）, operation control group （ group C, n = 3 ）. The group R was divided into eight groups randomly according to different time and temperature. Group S only has puncturation, group C didn＇t have puncturation and RA. Normal control group （ group N, n = 3 ） did not have CCI, puncturation or RA. After puncturation or RA cut off the ganglions of group R and S immediately and measured the diameter of damage. Group C and N were cut off the ganglions directly. The ganglions were putted into the formalin and observed the histological changes in the light microscope. Results Compare with group N, group S and group C had no differences both gross or in the light microscope. Under 65 ℃, there were no differences in group R either gross or in the light microscope. In group 70 ℃, there were slight lesions in gross and in the light microscope ; while in 75 ℃ and 80 ℃, group R had significant solidification and the diameter of damage were larger than group 70 ℃ （ P 〈 0. 01 ）, under light microscope, different degrees of damage appear, much higher the temperature and much longer the time, much serious damages of the neuron. Conclusion The temperature of 75 ℃ and 80 ℃ can produce semilunar ganglion cell degeneration, and the degree depends on the temperature and time of RA.