聚焦超声辐照豚鼠慢性湿疹模型的超微结构变化

目的探讨聚焦超声辐照豚鼠慢性湿疹模型豚鼠的皮肤超微结构改变。方法豚鼠32只，以2，4-二硝基氯苯（DNCB）为抗原，小剂量多次刺激豚鼠背部皮肤制备慢性湿疹模型。随机分为辐照组3组和对照组，辐照组以频率10MHz、声功率3W的连续波直接辐照模型皮肤真皮层，3组分别于辐照后1、7和14天取材，对照组不辐照立即取材，做透射电镜观察．结果辐照前可见表皮角质层增厚明显，成纤维细胞线粒体肿胀、粗面内质网扩张，细胞间隙加大；辐照后第1天和7天可见血管内皮细胞线粒体肿胀、微吞饮小泡数量多，成纤维细胞线粒体肿胀、粗面内质网扩张，但细胞间隙变小；第14天基本恢复正常。结论聚焦超声可用于治疗湿疹，有利于微血管和胶原纤维的增生与修复，可使患处上皮细胞及真皮组织的超微结构趋于恢复正常。

Ultrastructural Changes of Focused Ultrasound on Guinea Pig model of Chronic Eczema

Objective To investigate electron microscopic changes after focused ultrasound irradiation in guinea pig model of chronic eczema. Methods The chronic eczema models were prepared by using 2,4-dinitrochlorobenzene （DNCB） as an antigen to stimulate repeatedly the shaved sites on the back of guinea pigs. 32 guinea pigs were randomly divided into 4equal groups（3 irradiation groups and 1 control group）, irradiating chronic eczema models dermis in irradiation groups with ultrasound frequency 10 MHz, sound power 3W, We take samples on 1,7 and 14 days after irradiation. Results Before ultrasound irradiation ,the visible stratum corneum thickened apparently. The mitochondria of fi- broblast swelled. The rough endoplasmic reticulum expanded and the intercellular space increased. 1 and 7 days after ultrasound irradiation, endothelial cell of blood vessel exist mitochondria swelling and more micropinocytotic vesicle, fibroblast have mitochondria swelling and rough endoplasmic reticulum dilatation, and merely intercellular space became smaller. On the 14th day it returned to normal. Conclusion Focused ultrasound can be used to treat eczema. It is in favor of hypertrophy and rehabilitation of microvasculature and collagen fibrils,and the affected area of epithelial ceils and dermal tissue tends can return to normal.