板蓝根生药中蛋白质提取方法的比较

分别以40％乙醇、20％乙醇和0．02mol／L盐酸3种提取液来提取生药板蓝根中的蛋白质，并以Tris-HCl缓冲液为对照，用752紫外分光光度计测定各样品提取液的O．D值，计算蛋白质的含量：蛋白质浓度(mg／mL)=1．45D280-0．74D260，同时测定各样品的血凝活性，并对板蓝根中蛋白质含量与血凝活性的相关性进行了分析．实验结果表明：3种提取液对同一种板蓝根生药中蛋白质的提取量存在显著差异，其中以40％乙醇为提取液所测得的蛋白质含量最高，但蛋白质含量多少与血凝活性之间并无明显相关性．

A comparative study on different extraction methods on protein in Isatis indigotica Fort

The protein content in Isatis indigotica Fort is related to its amino acid content, and the amino acid content is related to its arginine, so the higher protein content in Isatis indigotica Fort has, the higher amino acid content does. Thus it is of great significance to find more effective extraction methods to research the medicinal value. In this experiment it has desiged three methods on different solutions to extract protein in Isatis indigotica Fort. , they are the ethyl alcohol of 40% , the ethyl alcohol of 20% and 0.02 mole hydrochloric acid, and taking Tris - HCl( pH = 7 ) for control group. Then using ultraviolet spectrophotom-eter752 to tested the protein content by O. D numerical value, protein concentration( mg/mL) = 1.45D280 - 0. 74D260. and to tested their hemagglutination activity. The results showed that the different extraction methods made great disparities. The method of using the ethyl alcohol of 40% is the best one, but the determination of hemagglutination activity indicated that the protein content was not marked positive correlation related with its hemagglutination activity.