| 利用小麦微卫星引物建立黑麦特异标记及其局限性 |
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| 引用本文： | 田茂洁,唐宗祥. 利用小麦微卫星引物建立黑麦特异标记及其局限性[J]. 西华师范大学学报(哲学社会科学版), 2009, 30(1): 17-20 |
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| 作者姓名： | 田茂洁 唐宗祥 |
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| 作者单位： | [1]西华师范大学生命科学学院,四川南充637009 [2]四川农业大学植物遗传育种省级重点实验室,四川雅安625014 |
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| 基金项目： | 国家自然科学基金资助项目(30671136);;四川农业大学校青年基金资助项目(00131300) |
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| 摘 要： | 用50对小麦SSR引物对13种黑麦基因组DNA进行扩增，只有Xgwm232，Xgwm260，Xgwm644这3对小麦SSR引物能扩增出黑麦特异的片段．这些黑麦特异片段主要集中在500bp，600bp，800bp和1000bp左右，并包含了启动子区域．然而这3对SSR引物都不能从所有13种黑麦中扩增出黑麦特异条带．这一结果表明尽管利用小麦微卫星引物能够开发黑麦特异DNA标记，但这类标记不是对所有黑麦都适用．利用某一物种的微卫星引物开发其近缘种属的特异DNA标记虽然可行，但不一定具有通用性．
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| 关 键 词： | 小麦 微卫星引物 黑麦 特异标记 |
The Development and Limitation of Rye-Specific Markers Using Wheat SSR Markers |
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| TIAN Mao-jie,TANG Zong-xiang. The Development and Limitation of Rye-Specific Markers Using Wheat SSR Markers[J]. Journal of China West Normal University:Natural Science Edition, 2009, 30(1): 17-20 |
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| Authors: | TIAN Mao-jie TANG Zong-xiang |
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| Affiliation: | 1.School of Life Science;China West Normal University;Nanchong 637009;China;2.State Key Laboratory of Plant Breeding and Genetics;Sichuan Agriculture University;Ya'an 625014;China |
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| Abstract: | Fifty wheat SSR markers were used to amplify genomic DNA of 13 Secale accessions.Only 3 SSR markers,Xgwm232,Xgwm260 and Xgwm644,could amplify rye-specific bands.Most of these bands are about 500bp,600bp,800bp and 1000bp in size and these DNA segments contain promoter regions.However,among the 3 wheat SSR markers,there was not a marker which can amplify rye-specific bands from all the 13 Secale accessions.These results indicate that wheat SSR markers can be used to develope rye-specific molecular markers,how... |
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| Keywords: | wheat SSR marker Secale cereale specific marker |
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