黄胫小车蝗配子发生时c-kit蛋白特异性表达

应用组织学、免疫组织化学和生物统计分析相结合的方法,对黄胫小车蝗(Oedaleus infernalisSauss.)配子发生期间原癌基因c-kit的特异表达进行了研究.结果表明:精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;卵子发生过程中,第1~6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄的发生逐渐消失.

The special expression of c-kit protein during gametogenesis of Oedaleus infernalis Sauss.

By using histology,immunohistochemistry and biostatistics,the c-kit special expression in those phases were studied by collecting Oedaleus infernalis Sauss.from the Siping area,Jilin province,China.The results showed that:(1)In spermatogenesis,there were different expression levels of c-kit protein in spermatogonia,primary spermatocytes,second spermatocytes and mature sperms,and the biggest positive granules were found at the end of spermary.(2)In oogenesis,there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes but the positive granules gradually disappeared with the following vitellogenesis.