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正常和细胞质雄性不育水稻线粒体DNA限制性内切酶的酶切图谱分析
引用本文:卓德艮,叶正祥,汪训明. 正常和细胞质雄性不育水稻线粒体DNA限制性内切酶的酶切图谱分析[J]. 复旦学报(自然科学版), 1988, 0(4)
作者姓名:卓德艮  叶正祥  汪训明
作者单位:湖南省农科院,复旦大学遗传学研究所,复旦大学遗传学研究所 研究生
摘    要:从N,cms-WA,cms-BT和cms-RL四种不同细胞质源的水稻中制备线粒体DNA。用限制性内切酶Pst Ⅰ,Hind Ⅲ和Xho Ⅰ等酶切线粒体DNA经琼脂糖凝胶电泳,发现水稻N,cms-WA,cms-BT和cms-RL细胞质确有不同的线粒体DNA,不同的保持系之间也存着一定的差异。水稻线粒体DNA最小分子的大小在195~245kb之间。

关 键 词:水稻  细胞质雄性不育  线粒体DNA  限制性内切酶。

RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL DNA FROM NORMAL AND THE CYTOPLASMIC MALE-STERILE RICE
Zhuo Degen,Ye Zhengxiang,Wang Xunming,. RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL DNA FROM NORMAL AND THE CYTOPLASMIC MALE-STERILE RICE[J]. Journal of Fudan University(Natural Science), 1988, 0(4)
Authors:Zhuo Degen  Ye Zhengxiang  Wang Xunming  
Affiliation:Institute of Genetics
Abstract:The rice mitochondrlal DNAs (mt-DNAs) were prepared from members of N(fertile), cms-WA, cms-BT and cms-RL groups. Restriction endonucleases Pst I,Hind III and Xho I were used to digest the mt-DNAs, and the fragments resultedwere run the gel-electrophoresis and compared. The results showed that they werequite distinct from each other and that mitochondrial DNAs from the NormalZhenxian 97 B and Nonghu 26 B Were different. The estimated minimum molecularsizes of rice mt-DNAs ranged from 195~245kb.
Keywords:rice  cytoplasmic male-sterility  mitochondrial DNA (mt-DNA)  restriction endonuclease.
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