蛇足石杉的组织培养初探

针对蛇足石杉资源分散，资源更新周期长，难以大规模开采的情况，初步探讨了蛇足石杉的组织培养技术.以蛇足石杉茎尖为外植体，分别在1/2MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L谷氨酰胺、MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L谷氨酰胺、1/2MS+2 mg/L谷氨酰胺/无激素、MS+2 mg/L谷氨酰胺/无激素等4种培养基中培养.结果显示:较为适宜的初代培养基为2号培养基,即MS+0.05 μmol/L IBA+1.4μ mol/L KT+2 mg/L谷氨酰胺.在组织培养工作中，污染问题比较严重，本实验中，采用了以下2种方法对外植体进行消毒：（1）70%酒精浸泡1 min→5% NaOCl浸泡1 min→7%H₂O₂浸泡10 min;（2）0.1%升汞液灭菌3’ →无菌水冲洗5次.结果显示第2种方法比较理想.

Tissue Culture of Huperzia Serrata

In this study,tissue culture was explored to solve the propagate problem in the production with Huperzia serrata(Thunb)Trev.The stem and shoot-tips were used as explants to induce callus.These explants are cultured in four culture mediums:(1) 1/2MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine;(2) MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L pirydoxine;(3) 1/2MS+2 mg/L pirydoxine;(4) MS+2 mg/L pirydoxine.The results show that the suitable element compositions to induce callus is:MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine.During tissue culture,fungal pollution is very serious,so the surface disinfection is important.There are two variants of surface sterilization.In the first variant shoot-tips were soaked in 70% ethyl alcohol (C₂H₅OH) for 1 min,5% sodium hypochlorite (NaOCl) for 1 min and then in 7% hydrogen peroxide (H₂O₂) for 10 min;the second variant were soaked in HgCl for 3 min.The result shows that the most suitable procedure for surface sterilization of explants is the second one.