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| 核酸水解酶及酶解法生产核苷酸研究进展 | |
| 引用本文： | 何义进,华洵璐,匡群,史济筠,邹三元,张一平.核酸水解酶及酶解法生产核苷酸研究进展[J].辽宁大学学报(自然科学版),2012,39(2):110-117. |
| 作者姓名： | 何义进 华洵璐 匡群 史济筠 邹三元 张一平 |
| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081 2. 江苏省苏微微生物研究有限公司,江苏无锡,214063 3. 江苏省宜兴市天石饲料有限公司,江苏宜兴,214258 |
| 基金项目： | 2010年科技部农业科技成果转化资金项目，2008年科技部农业科技成果转化资金项目，农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金 |
| 摘 要： | 综述了目前主要核酸水解酶的种类、来源、蛋白质特性和生产方法;介绍了目前酶解法生产核苷酸的几种主要方法,比较了各种方法的优缺点及工业化应用前景;简单综述了水解液中四种核苷酸的分离提取方法;探讨了酶解法生产核苷酸的未来研究方向.结论为酶解法的关键是必须通过广泛采用现代高新生物技术来降低生产成本. |
| 关 键 词： | 核酸 核苷酸 磷酸二酯酶 核酸酶P1 固定化 超滤 盐析 纯化 |
The Development of Studies on Nucleic Acid Hydrolase and Its Application in the Nucleotide Production |
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| HE Yi-jin , HUA Xun-lu , KUANG Qun , SHI Ji-jun , ZOU San-yuan , ZHANG Yi-ping.The Development of Studies on Nucleic Acid Hydrolase and Its Application in the Nucleotide Production[J].Journal of Liaoning University(Natural Sciences Edition),2012,39(2):110-117. | |
| Authors: | HE Yi-jin HUA Xun-lu KUANG Qun SHI Ji-jun ZOU San-yuan ZHANG Yi-ping |
| Institution: | 1.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China; 2.Jiangsu Limited Company of Suwei Microbiology,Wuxi 214063,China; 3.Jiangsu Yixing Feedstuff Company of Tian Shi,Yixing 214258,China) |
| Abstract: | In this paper,the kinds、sources、protein characters and the manufacture methods of nucleic acid hydrolase were summarized;the mainly nucleotide production methods with the hydrolase were indroduced,and the advantages、defects、industry prospects of these methods were also be comparised;the separation and extraction methods of four single nucleotides in the hydrolyzed liquid were simply summarized;the future studies trends of nucleotides production by enzyme method were also be discussed.The Conclusion was that the enzyme methods must lowered the production cost through the widely using of modern biotechnology. |
| Keywords: | nucleic acid nucleotide phosphodiesterase nucleaseP1 immobilization ultrafiltration salting out purification |
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