| 毛细管电泳-安培法测定酵母菌中的RNA |
| |
| 引用本文： | 陈相,王清江,丁飞,吕金,何品刚,方禹之.毛细管电泳-安培法测定酵母菌中的RNA[J].华东师范大学学报(自然科学版),2006,2006(2):7-12. |
| |
| 作者姓名： | 陈相 王清江 丁飞 吕金 何品刚 方禹之 |
| |
| 作者单位： | 华东师范大学,化学系,上海,200062;华东师范大学,化学系,上海,200062;华东师范大学,化学系,上海,200062;华东师范大学,化学系,上海,200062;华东师范大学,化学系,上海,200062;华东师范大学,化学系,上海,200062 |
| |
| 基金项目： | 上海市科委自然科学基础研究项目 |
| |
| 摘 要： | 报道了通过毛细管电泳-安培检测法测定腺嘌呤(A)和鸟嘌呤(G)来间接测定酵母菌中核糖核酸含量的方法.探讨了电极电位、运行缓冲溶液、分离电压及进样时间等对毛细管电泳分离-安培检测的影响,得到了较为优化的测定条件.在30 mmol/L 的硼酸盐缓冲液(pH 9.0)中,上述两组分可在18 min 内完全分离.腺嘌呤和鸟嘌呤的检测限分别为2.0×10-7mol/L和1.7×10-7 mol/L.该法具有较好的重现性和灵敏度，可以用于食品中酵母菌中核糖核酸的快速测定.
|
| 关 键 词： | 毛细管电泳 安培检测 酵母菌 核糖核酸 |
| 文章编号： | 1000-5641（2006）02-0007-06 |
| 收稿时间： | 2005-03 |
| 修稿时间： | 2005-03 |
Determination of Content of RNA in Yeast by Capillary Zone Electrophoresis with Amperometric Detection |
| |
| CHEN Xiang,WANG Qing-jiang,DING Fei,L Jin,HE Pin-gang,FANG Yu-zhi.Determination of Content of RNA in Yeast by Capillary Zone Electrophoresis with Amperometric Detection[J].Journal of East China Normal University(Natural Science),2006,2006(2):7-12. |
| |
| Authors: | CHEN Xiang WANG Qing-jiang DING Fei L Jin HE Pin-gang FANG Yu-zhi |
| |
| Institution: | Department of Chemistry, East China Normal University, Shanghai 200062, China |
| |
| Abstract: | The indirect determination of the content of RNA in yeast by analyzing adenine (A) and guanine (G) with capillary electrophoresis with amperometric detection (CE-AD) was reported. The effects of potential of working electrode, running buffer, separation voltage and injection time on the CE-AD determination were investigated. Adenine and guanine could be well separated in a 30 mmol/L borate buffer (pH 9. 0) within 18 min under the optimum conditions. Their detection limits of adenine (S/ N = 3) of 2.0 ×10-7 mol/ L and 1.7 ×10 -7mol/ L, respectively. The experimental results showed that this method is of high sensitivity and high repeatability, and potential to be used in the quick analysis of yeast in foods. |
| |
| Keywords: | capillary electrophoresis amperometric detection yeast ribonucleic acid(RNA) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《华东师范大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《华东师范大学学报(自然科学版)》下载免费的PDF全文 |
|
