| 时间分辨多光谱技术研究 |
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| 引用本文： | 郭宝平,田劲东,屈军乐,牛憨笨.时间分辨多光谱技术研究[J].中国基础科学,2004,6(6):11-14. |
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| 作者姓名： | 郭宝平 田劲东 屈军乐 牛憨笨 |
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| 作者单位： | 深圳大学光电子学研究所 |
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| 基金项目： | 国家自然科学基金资助项目 (No .60 1 380 1 0 ) |
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| 摘 要： | 本文讨论了具有时间分辨的多光谱探测技术 ,并通过结合钛宝石锁模激光器、荧光显微、高重复频率皮秒扫描相机等技术 ,研制出一套基于皮秒同步扫描相机的五维双光子激发荧光多参数复合显微成像系统 ,实现了细胞层次上的五维测量 ,并可方便地实现多种功能成像 ,例如荧光强度、荧光寿命和荧光光谱成像
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| 关 键 词： | 高重复频率皮秒扫描相机 荧光显微 双光子荧光多光谱显微成像系统 |
Research on the Time Resolved Multi-spectral Detection Technology |
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| Guo Baoping Tian Jindong Qu Junle Niu Hanben Institute of Optoelectronics,Shenzhen University,Shenzhen.Research on the Time Resolved Multi-spectral Detection Technology[J].China Basic Science,2004,6(6):11-14. |
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| Authors: | Guo Baoping Tian Jindong Qu Junle Niu Hanben Institute of Optoelectronics Shenzhen University Shenzhen |
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| Institution: | Guo Baoping Tian Jindong Qu Junle Niu Hanben Institute of Optoelectronics,Shenzhen University,Shenzhen 518060 |
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| Abstract: | In this paper, the time resolved multi-spectral detection technology is used in the fluorescence microscopy imaging system. A five-dimensional two-photon fluorescence microscopy imaging system is developed to realize five-dimensional fluorescence measurement on cellular level. The system is based on mode-locked Ti:sapphire laser, fluorescence microscopy and synchroscan streak camera. This fluorescence microscopic system can be used to measure multi-parameter including fluorescence intensity, fluorescence lifetime and fluorescence spectrum. |
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| Keywords: | high repetition rate trigger mode streak caera fluorescence microscopy imaging system two-photon fluorescence microscopy imaging system |
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