| 家兔中性粒细胞防御素的纯化及其对念珠菌抗菌活性的研究 |
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| 引用本文： | 蒋献,冉玉平,黄宁,吴琦,周光平.家兔中性粒细胞防御素的纯化及其对念珠菌抗菌活性的研究[J].四川大学学报(自然科学版),2001,38(2):239-242. |
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| 作者姓名： | 蒋献 冉玉平 黄宁 吴琦 周光平 |
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| 作者单位： | 1. 华西医科大学附属第一医院皮肤性病科,
2. 华西医科大学基础医学院感染免疫研究室, |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳分离纯化兔中性粒细胞防御素，兔中性粒细胞防御素NP2具有抗白念珠菌M1012R，INH A-207，乳酒念珠菌，热带念珠菌和高里氏念珠菌等菌的活性，具抗菌活性随防御素浓度的升高而增强。
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| 关 键 词： | 兔中性粒细胞及御素 念珠菌 抗菌活性 分离纯化 内源性抗生素肽类分子 皮肤真菌感染性疾病 |
| 文章编号： | 0490-6756(2001)02-0239-04 |
IN VITRO ANTIFUNGAL
ACTIVITY OF NEUIROPHH DEFENSINS AGAINST SOME CANDIDA Spp. |
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| JIANG Xian,RAN Yu-ping,HUANG Ning,WU Qi,ZHOU Guang-Pi.IN VITRO ANTIFUNGAL
ACTIVITY OF NEUIROPHH DEFENSINS AGAINST SOME CANDIDA Spp.[J].Journal of Sichuan University (Natural Science Edition),2001,38(2):239-242. |
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| Authors: | JIANG Xian RAN Yu-ping HUANG Ning WU Qi ZHOU Guang-Pi |
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| Abstract: | To investigate the role and mechanism of neutrophil defensins against some Candida spp. Neutrophil defensins were purified by polyacrylamide gel electrophoresis (SDS-PAGE), and neutrophil defensin NP2 were tested for its antifungal activity against C andida albican M1012R, NIH A-207, Candida kefyr, Candida tropicalis and Candida guilliermondii. NP2 was successfully isolated and purified by AU-PAGE, and demonstrated concentration-dependent killing of Candid albican M1012R, NIH A-207, Candida kefyr, Candida tropicalis and Candida guilliermondii in vitro. Acid urea polyacrylamide gel electrophoresis is simple and convient in isolating and purifying protein and polypeptides, neutrophil defensin NP2 exerted potent antifungal activity against Carndida spp. |
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