| 中国野猪核DNA SANDX-2位点遗传变异分析 |
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| 引用本文： | 霍爱玲,张晨岭,李崇奇,陈建琴,周开亚,常青.中国野猪核DNA SANDX-2位点遗传变异分析[J].南京师大学报,2007,30(3):79-86. |
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| 作者姓名： | 霍爱玲 张晨岭 李崇奇 陈建琴 周开亚 常青 |
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| 作者单位： | [1]南京师范大学遗传资源研究所,江苏南京210097 [2]海南医学院基础医学部,海南海口571101 [3]江苏教育学院生物系,江苏南京210016 |
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| 基金项目： | 江苏省生物多样性与生物技术重点实验室基金 |
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| 摘 要： | 研究测定了89个中国野猪和1个家猪未知功能核基因SANDX-2位点的序列.在序列比对后长510 bp左右,发现有2处4种类型碱基缺失,30个位点发生碱基替换,共定义了19种单元型.在野猪中共发现18种单元型,其中7种只在某一地方种群中发现,11种为地方种群间共享单元型.用邻接法基于核苷酸 Kimura 2-parameter模型构建系统发生树中,19种单元型被明显分为2大枝.尽管各枝得到的Bootstrap值均较低,且各地方种群中出现的单元型散布在不同的进化枝中,但单元型在野猪亚种内的分布已出现了一定的地理分布格局.若不考虑碱基的插入/缺失,用TCS构建19种单元型网络表明,HAP2单元型为一古老单元型,其他为衍生单元型.
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| 关 键 词： | 中国野猪 SANDX-2位点 遗传变异 中国 野猪 遗传变异分析 Wild Chinese Nuclear Variation 衍生 网络表 分布格局 地理 亚种 Bootstrap 系统发生树 模型 Kimura 核苷酸 邻接法 单元型 碱基替换 |
| 文章编号： | 1001-4616（2007）03-0079-08 |
| 收稿时间： | 2006-03-05 |
| 修稿时间： | 2006-09-11 |
Genetic Variation of Anonymous Nuclear DNA SANDX-2 in Chinese Wild Boars (Sus scorfa) |
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| Huo Ailing,Zhang Chenling,Li Congqi,Chen Jianqin,Zhou Kaiya,Chang Qin.Genetic Variation of Anonymous Nuclear DNA SANDX-2 in Chinese Wild Boars (Sus scorfa)[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(3):79-86. |
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| Authors: | Huo Ailing Zhang Chenling Li Congqi Chen Jianqin Zhou Kaiya Chang Qin |
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| Institution: | 1. Genetic Resource Institute, Nanjing Normal University, Nanjing 210097, China;2. Department of Basic Medical Science, Hainan Medical College, Haikou 571101, China;3. Department of Biology, Jiangsu Institute of Education, Nanjing 210016, China |
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| Abstract: | The anonymous nuclear DNA SANDX - 2 locus of 89 individuals of Chinese wild boars and a domestic pig were amplified and sequenced for studying the genetic variability. A total of 510 bp were obtained after aligned, including 4 indels and 30 substitutions. Eighteen haplotypes were defined in Chinese wild boars, among which 7 haplotypes were observed only in one local population, and 11 haplotypes were shared by two or more local populations. The phylogenetic tree constructed by the neighbor-joining method based on Kimura 2 - parameters model divided the haplotypes into two distinct clades. Although the trees were not supported by high bootstrap value, and the haplotypes in different population were located in different clades, there are geographical distribution patterns in the Chinese wild boars. Ignoring the gaps in the sequences, the nesting cladogram of 19 haplotypes suggested that HAP2 may be ancestral haplotype. |
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| Keywords: | Chinese wild boar SANDX- 2 genetic variation |
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