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The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X |
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| Authors: | Bentley D R Deloukas P Dunham A French L Gregory S G Humphray S J Mungall A J Ross M T Carter N P Dunham I Scott C E Ashcroft K J Atkinson A L Aubin K Beare D M Bethel G Brady N Brook J C Burford D C Burrill W D Burrows C Butler A P Carder C Catanese J J Clee C M Clegg S M Cobley V Coffey A J Cole C G Collins J E Conquer J S Cooper R A Culley K M Dawson E Dearden F L Durbin R M de Jong P J Dhami P D Earthrowl M E Edwards C A Evans R S Gillson C J Ghori J Green L Gwilliam R Halls K S Hammond S Harper G L Heathcott R W Holden J L Holloway E Hopkins B L Howard P J Howell G R Huckle E J Hughes J |
| Affiliation: | The Sanger Centre, Hinxton, Cambridge, UK. drb@sanger.ac.uk |
| Abstract: | We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones. |
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