| 转海蓬子Na+/H+逆向运输蛋白基因番茄的耐盐性研究 |
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| 引用本文： | 张桂和,郭建春,叶妙水. 转海蓬子Na+/H+逆向运输蛋白基因番茄的耐盐性研究[J]. 贵州科学, 2007, 25(2): 47-50,77 |
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| 作者姓名： | 张桂和 郭建春 叶妙水 |
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| 作者单位： | 1. 海南大学海洋学院,海南,海口,570228
2. 中国热带农业科学院热带作物生物技术国家重点实验室,海南,海口,571101 |
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| 基金项目： | 海南省教育厅资助项目 , 海南省自然科学基金 , 中国热带农业科学院资助项目 |
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| 摘 要： | 以转海蓬子Na /H 逆向运输蛋白基因(Nhap)的番茄(Lycopersicon esculentum Mill.)阳性植株和对照植株为材料,测定叶片相对含水量、质膜透性、K /Na 比值和叶绿素含量4项耐盐指标.结果表明:在盐胁迫条件下,转基因植株的相对含水量、 K /Na 比值和叶绿素含量明显高于对照植株,而叶片质膜透性明显低于对照植株,说明Nhap基因已经在番茄转化植株体内表达,提高了转基因植株的耐盐性.
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| 关 键 词： | Nhap基因 转基因番茄 耐盐指标 |
| 文章编号： | 1003-6563（2007）02-0047-04 |
| 收稿时间： | 2006-11-29 |
| 修稿时间： | 2006-11-29 |
RESEARCH ON SALT-TOLERANCE OF TOMATO TRANSFORMED BY NHAP GENE FROM SALICORNIA BIGELOVII TORR |
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| ZHANG Gui-he,GUO Jian-chun,YE Miao-shui. RESEARCH ON SALT-TOLERANCE OF TOMATO TRANSFORMED BY NHAP GENE FROM SALICORNIA BIGELOVII TORR[J]. Guizhou Science, 2007, 25(2): 47-50,77 |
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| Authors: | ZHANG Gui-he GUO Jian-chun YE Miao-shui |
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| Affiliation: | 1. Ocean College of Hainan University, Haikou 570228, China; 2. State Key Laboratory of Tropical Crops Bioteehnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
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| Abstract: | Tomato Plants transformed by Nhap(Na+/H+ antiporter) gene from Salicornia Bigelovii Torr and control plants were tested using several salt-tolerance index such as relative leaf water content,plasma membrane permeability,rauio of K+/Na+ and content of chlorophyll.The results show that the transgenic tomato plants have higher relative leaf water content,ratio of K+/Na+ and chlorophyll content,and lower plasma membrane permeability than control plants under salt-stress conditions,which proves that Nhap gene has functioned in transgenic tomato plants and their salt-tolerance has been increased. |
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| Keywords: | Nhap gene transgenic tomato plants salt - tolerance index |
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