西沙野生诺尼叶片内生菌的分离与初步鉴定

主要研究西沙群岛野生诺尼叶片中可培养内生菌的多样性。利用常规平板分离方法进行菌株分离。通过测定真菌核糖体基因翻译间隔序列(ITS序列)和细菌16S r DNA基因序列,结合系统发育研究对所分离菌株进行鉴定分析。共分离到32株内生菌,分为19种。其中2种霉菌,共2株,分别属于炭层菌属(Nemania sp.)和毛壳菌属(Chaetomium sp.);细菌17种,共30株,分别属于芽孢杆菌属(Bacillus sp.)、赖氨酸芽孢杆菌属(Lysinibacillus sp.)、微球菌属(Micrococcus sp.)、土地芽孢杆菌属(Terribacillus sp.)、肠杆菌属(Enterobacter sp.)、黄单胞菌属(Xanthomonas sp.)、黄杆菌属(Flavobacterium sp.)等7个已知属,其中芽孢杆菌属(Bacillus sp.)为绝对优势属,共20株,11种。

Isolation and Identification of Endophytes in Wild Noni Leaves from Paracel Islands

The diversity of culturable endophytes in wild Noni leaves from Paracel Islands was discussed in this study. Strains were isolated by using conventional tablet separation methods. The isolates were identified by the fungal internal transcribed spacer 1 and 2 (ITS gene) amplification and the bacterial 16S rDNA amplification, combined with phylogenetic analysis. Total 32 endophytes were isolated and identified as 19 types. Two types of mold were Chaetomium sp. and Nemania sp. Seventeen types of bacteria (30 strains) belonged to 7 genera Bacillus sp., which were Lysinibacillus sp., Micrococcus sp., Terribacillus sp., Enterobacter sp., Xanthomonas sp., Flavobacterium sp., and Bacillus sp.. Bacillus sp. was the dominant genus among the endophytic bacte, which had 20 strains divided into 11 types.