| 拟南芥VHA-c3基因的特异性表达和调节 |
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| 引用本文： | 徐萍,李小方,曾卫军,王水平.拟南芥VHA-c3基因的特异性表达和调节[J].华东师范大学学报(自然科学版),2006,2006(2):98-104,111. |
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| 作者姓名： | 徐萍 李小方 曾卫军 王水平 |
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| 作者单位： | 华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062;华东师范大学,生命科学学院,上海,200062 |
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| 摘 要： | 利用RT-PCR技术检测VHA-c基因在拟南芥中的表达，结果表明VHA-c3基因在拟南芥的果荚、花、叶、茎和根中都有表达，但是，在叶中的表达量远远高于其它的组织.以GUS基因作为报告基因构建了不同长度的VHA-c3基因启动子缺失突变体，利用农杆菌介导的瞬时表达系统检测GUS基因的表达，研究发现在VHA-c3基因起始密码子上游2812-2 234 bp之间的区域內存在着控制VHA-c3基因高表达的转录调控元件.
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| 关 键 词： | V-ATPase c亚基 VHA-c3基因 启动子 |
| 文章编号： | 1000-5641（2006）02-0098-07 |
| 收稿时间： | 2005-04 |
| 修稿时间： | 2005-04 |
Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese) |
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| XU Ping,LI Xiao-fang,ZENG Wei-jun,WANG Shui-ping.Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)[J].Journal of East China Normal University(Natural Science),2006,2006(2):98-104,111. |
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| Authors: | XU Ping LI Xiao-fang ZENG Wei-jun WANG Shui-ping |
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| Institution: | School of Life Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | Gene expression of VHA c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium mediated transient expression system. It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis element for leafspecific expression between 2 812-2 234 bp region. |
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| Keywords: | V-ATPase c subunit VHA c3 promoter |
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